Description
The world's first commercially available single-cell electroporator represents the convergence of biophysics, cell biology, and molecular biology. The Axoporator® 800A Electroporation System, a micropipette-based molecule delivery system, uses voltage pulses to cause the transient formation of membrane pores through which membrane-impermeant molecules and ions can enter the cell. The mechanism is the same as that used for the bulk electroporation of suspended cells-dielectric breakdown of the membrane by a voltage pulse. However, single-cell electroporation offers distinct advantages over bulk electroporation: individual cells can be targeted for specific modification. Whether you're using isolated cells, tissue slices, or an intact preparation, this technique allows you to focus on an individual cell.
Features - Ready to use. No computer is necessary. A microscope, a micromanipulator, and a source of micropipettes are everything needed to complete the system.
- Small footprint for easy positioning near the microscope
- Three different pulse types, configurable in either polarity, ensure the optimal waveform for your application
- Pulse trains for greatest flexibility of the voltage waveform
- Automatic and manual counters simplify tracking the number of electroporation commands.
- Voltage range up to 100 V provides the optimal voltage for single-cell electroporation, which can be up to several tens of volts in some preparations.
- Available AP-1A-0.1MU headstage with current passing range up to ±100 µA
- Built-in audio monitor with headphone connector changes tone as you near a cell and make contact.
- Resistance display, in combination with audio monitor, provides a useful tool for searching out cells in a tissue slice without visual control.
- Stand-alone or computer-controlled pulse start for greatest flexibility and synchronization with electrophysiology experiments. See Related Products section for more details
- Foot switch to trigger pulse start while your hands are busy
- Sync output BNC for synchronization of a third-party recording system
- User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference.